Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms

Authors

  • A. Ashtari
  • A.R. Abtin
  • A.R. Homayounimehr
  • M.A. Bayatzadeh
Abstract:

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 main provinces of Iran (Tehran, Markazi and Qazvin), with clinical signs of the disease. Total of 43 samples were cultured in PPLO broth media supplemented for M. synoviae isolation. The bacteria DNAs were extracted by phenol/chloroform method and the PCR assay amplifying the conserved region of 16S rRNA gene was applied for the detection of Mycoplasma genus in 163bp fragment and M. synoviae in 207bp fragment from culture as same as in clinical samples. Of the 43 swabs 28(65.1%) yielded one of the potentially pathogenic mycoplasmas evaluated for using PPLO agar culture diagnostic method, and 33(76.8%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and 24(55.9%) of the swabs yielded M. synoviae for using M. synoviae PCR as diagnostic method. In this study we had observed the highest quantity of M. synoviae infections in broiler chicken with PCR test. In conclusion, PCR is a more rapid, effective, sensitive and inexpensive method than the standard culture technique, that could be used as an alternative method for traditional culture and showed the real number of the M. synoviae contaminated broiler chicken farms.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

application of culture and polymerase chain reaction (pcr) methods for isolation and identification of mycoplasma synoviae on broiler chicken farms

mycoplasma synoviae (m. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. this study was designed to detect m. synoviae through culture isolation and polymerase chain reaction (pcr) assay to demonstrated the involvement of m. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

full text

Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran

Contagious agalactia (C.A) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from...

full text

Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods

Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 47...

full text

Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from affected sheep to Contagious agalactia of Khuzestan province, Iran

Mycoplasma agalactiae (M. agalactiae) is one of the main causes of contagious agalactia, an infectious syndrome of sheep and goats in Khuzestan province –southwest of Iran that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. This study was carried out to isolation and identification of M. agalactiae with culture and polymeras...

full text

Isolation and Identification of Mycoplasma agalactiae by Culture and Polymerase Chain Reaction Methods in the Sheep Herds in Guilan Province, Iran

Contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. The disease is rapidly spread by the contact of the infected animals with the healthy ones. Domestic sheep and goats of both sexes can be infected at an equivalent frequency. Most of the researchers use culture and molecular ...

full text

isolation and identification of mycoplasma agalactiae by culture and polymerase chain reaction (pcr) from sheep of qom province, iran

contagious agalactia (c.a) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. mycoplasma agalactiae (m. agalactiae) is the main cause of the disease in sheep. the aim of this study was isolation and identification of m. agalactiae with culture and polymerase chain reaction (pcr) assay f...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 66  issue 2

pages  87- 94

publication date 2011-11-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023